On-line solid-phase extraction high-performance liquid chromatography-tandem mass spectrometry for t... Zwitterionic poly(2-oxazoline)s as promising candidates for blood contacting applications. Dans ce contexte, le travail présenté ici s’est focalisé sur l’écologie chimique du dinoflagellé benthique toxique Ostreopsis cf. Also, we are going to discuss the possible ways of terrorist attacks by using biological or chemical agents, starting from farms, processing, transportation, distribution and other stages of the sophisticated and long food chain processing and services. Bloom developed when the seawater temperature was decreasing. 2010 - 2010 Suivi des efflorescences d'une microalgue benthique et toxique Ostreopsis ovata. ovata under different light and temperature conditions, Molecular phylogeny, morphology and toxigenicity of Ostreopsis cf. Both of these dinoflagellates produce palytoxin (PTX)-like toxins that are powerful vasoconstrictors in mammals. ovata (NW Mediterranean Sea): Structural insights by LC-high resolution MS, Complex Toxin Profile of French Mediterranean Ostreopsis cf. Marine HABs are diverse in terms of causative organisms,which are usually cyanobacteria and planktonic or benthic microalgae, although the term may also be applied to harmful blooms of macroalgae (Glibert With 10 μ L of serum or plasma, the lower limit of quantification for the assay (<20% CV) was 71 μ g/mL. Virus, bactéries et protistes pathogènes du phytoplancton le rôle insoupçonné des parasites dans le fonctionnement des écosystèmes aquatiques Etude de Vulcanodinium rugosum (Dinoflagellé producteur de pinnatoxines) se développant dans la lagune méditerranéennede l’Ingril. Previous studies showed poor PLTX recoveries with a dramatic decrease in PLTX yield throughout each purification step. En Turquie, le jeu dangereux d'Erdogan. Ostreopsis ovata Detoxification of a naturally toxic mussel populations from an area affected by O. cf. Aquasud : l'UCPA reconduite pour 15 ans. Role of temperature and nutrients on the growth and toxin production of Prorocentrum hoffmannianum (Dinophyceae) from the Florida Keys, Key Questions and Recent Research Advances on Harmful Algal Blooms in Benthic Systems, Allelopathic interactions between the benthic toxic dinoflagellate Ostreopsis cf. ovata during bloom events, AFLP reveals intraspecific variations in geographically diverse Ostreopsis cf. Sample pretreatment is usually the most complicated step in the analytical process and covers both cleaning and pre-concentration. nov. (Dinophyceae), a new benthic toxic Ostreopsis species from the eastern Mediterranean Sea, Toxin profile of Ostreopsis cf. ovata on Mytilus galloprovincialis and Paracentrotus lividus: Toxin accumulation and pathological aspects, Human impact in Mediterranean coastal ecosystems and climate change: emerging toxins, In Vitro Cell Sensitivity to Palytoxin Correlates with High Gene Expression of the Na+/K+-ATPase β2 Subunit Isoform, Taxonomy and toxicity of a bloom-forming Ostreopsis species (Dinophyceae, Gonyaulacales) in Tahiti island (South Pacific Ocean): one step further towards resolving the identity of O. siamensis, Ecologie chimique du dinoflagellé toxique Ostreopsis cf. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. This study constitutes the first characterization of a calcium complex with palytoxin and, as such, is expected to support the investigation of the toxin molecular bioactivity. We identified two specific regions of palytoxin where Ca2+ is preferentially coordinated. ovata and in Structural Features of the Produced Ovatoxins, Toxin and Growth Responses of the Neurotoxic Dinoflagellate Vulcanodinium rugosum to Varying Temperature and Salinity, The toxic benthic dinoflagellates of the genus Ostreopsis in temperate areas: a review, Ostreopsis cf. In 2011, human poisoning cases caused by the consumption of blue humphead parrotfish occurred in Miyazaki and Tokyo. The applicability of several extraction and clean-up techniques employed in the analysis of foodstuffs, especially milk and egg samples, is also thoroughly discussed. Method comparison with immunoassay revealed significant isoform-specific effects in the immunoassay. Experiments will be carried to identify their chemical structures, mechanism of action and risk assessment. et al. C'est donc avec dépit que j'apprends ce matin (09/09/2021) l'interdiction de baignade, de jeux nautiques et bien entendu de surf en raison de l'algue toxique "Ostreopsis ovata" et ce de Saint-Jean de Luz à Lacanau. The amino-acidic reagent 6-aminoquinolyl-N-hydroxisuccinimidyl carbamate (AccQ) was used for fluorescence labelling followed by LC–FLD. In subsequent studies in the same area, PLTXs levels in contaminated sea urchins ranged from 103 to 423 μg kg -1 of whole flesh, with an average of 223 μg kg -1 revealing a high variability of contamination levels between individual organisms, while in the case of contaminated sea breams, PLTX levels ranged between 33 and 152 μg kg -1 in the whole flesh, ... Palytoxin (PLTX) is one of the most potent marine toxins, originally identified in Palythoa zoanthids corals [1]. The wide applicability of the method was suggested. Based on the observed association between O. cf. ovata sampled week 30 in 2011: ( A ) Ostreopsis proliferation between the surface and 5 m depth on brown macroalgae Padina pavonica ; and ( B ) Toxin concentration and profile of Ostreopsis cf. It has been demonstrated that the increase of cytosolic free Ca2+ concentration gives rise to downstream events ultimately leading to cell death. S. algae is adapted to a wide range of temperatures (4°C, 25°C, 37°C, and 42°C) and salinity. (Dinophyceae) from Malaysia, Polymerase Chain Reaction in Detection of Gymnodinium mikimotoi and Alexandrium minutum in Field Samples from Southwest India, Potentially toxic epiphytic dinoflagellate assemblages on macroalgae in NW Mediterranean, Geometric models for calculating cell biovolume and surface area for phytoplankton, The CLUSTAL_X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools, First record of Ostreopsis cfr. Crédit image : Smithsonian Institution. Although PLTX was quite stable in 0.2% acetic acid solutions, upon exposure to stronger acids (pH < 2.66), degradation products were observed, among which a PLTX methyl-ester was identified. the gonads, were not toxic in the few cases of mild toxicity detected in the other sea urchin tissues, in agreement with studies showing the accumulation of toxins in the digestive tract (Amzil et al., 2012; ... For instance, in the Mediterranean coasts of France, maximum levels of PLTXs contamination in tissues of edible organisms tested by LC-MS/MS were higher than the EFSA proposed level of 30 μg kg -1 in the sea urchin Paracentrotus lividus, the redmouthed rock shell Stramonita haemastoma, the warty crab Eriphia verrucosa and the flathead mullet Mugil cephalus, with the last one presenting the highest toxin level of the study being 392.2 μg kg -1 for the sum of OVTX-a and PLTX per kg of digestive tube [191]. Its intravenous lethality (LD50), which ranges from 0.025 µg/kg in the rabbit to 0.45 µg/kg in the mouse (2), is exceeded only by certain proteins and polypeptides (3). 2014). Significant hemolysis interfered with quantification. Thus, an approximate amount of 10 mg of PLTX-group toxins may be . ovata blooms and they were analyzed by both PCR assays and LC-HRMS technique. Engagée dans une démarche concertée avec l'ensemble des acteurs locaux, Surfrider Europe souhaite améliorer la communication liée aux résultats eux-mêmes mais également les enquêtes initiées en cas de . However, this research is challenged by technical limitations to conduct ecologically realistic and harmonized studies where organisms can be in direct contact with Ostreopsis cells, their mucus and/or the released extracellular toxic compounds. A better assessment of the vertical distribution of toxic microalgae could be very useful for a suitable management and an effective anticipation of HAB events. Extraction with MeOH/H2O 8:2 provided the best results in terms of accuracy and matrix interference on LC-MS/MS detection of palytoxin. L'UCPA conserve la gestion et l'animation du Complexe aquatique municipal Aquasud en délégation de service public pour les 15 prochaines années. Positive ion spectra allowed deduction of hydroxyl positions based on the conjugated polyene structures produced, while the negative ion spectra allowed assignments of cleavage sites of C-C bonds. Une micro-algue toxique sur les plages de la Côte basque française. In summer 2005 along the urbanized Genoa coastline (Ligurian Sea, North western Mediterranean Sea), local first aid stations treated about 200 people, who all showed similar symptoms following exposure to marine aerosols. École Doctorale Végétal-Environnement-Nutrition-Agroalimentaire-Mer VENAM (2014) An overview of the main foodstuff sample preparation technologies for tetracycline residue determina... New Tools for Microbiological Risk Assessment, Risk Management, and Process Validation Methodology. usines de traitement d . ovata qui est le facteur à l’origine des mortalités de masse d’invertébrés décrites en milieu naturel. have been recently reported along the Mediterranean coasts of Spain, France, Italy, and Greece posing serious risks to human health. Investigators at the University of Hawaii were led to P. toxicawhen they followed up the lead provided by the entry limu-make-o-Hana(the deadly seaweed of Hana) in the Hawaiian-English Dictionary (5). (Dinophyceae) in New Zealand: Temporal and regional distribution in the upper North Island from 1995 to 1997, PROSEQ: A software for preparation and evolutionary analysis of DNA sequence data sets, Comparative study of Gymnodinium mikimotoi and Gymnodinium aureolum, comb. Ovatoxin-a is the main toxin produced by Ostreopsis ovata, a benthic dinoflagellate that has bloomed massively across the Mediterranean basin over the past years, inflicting both human and environmental suffering. ovata in this temperate area has been linked to global changes and its consequences such as the increase of temperature or light intensities. les champignons vénéneux et la plupart des toxines sont également naturels . ovata impacte négativement la croissance des diatomées compétitrices, tandis que ces dernières inhibent en retour sa croissance et son efficacité photosynthétique. Cécile a 8 postes sur son profil. With the aim of replacing current biological assays, such as the mouse or hemolytic assays, we investigated using the Neuro-2a neuroblastoma cell line to detect palytoxin and related compounds. PDF | On Feb 27, 2013, Waffa Iddir-Ihaddaden and others published [Health impact of unicellular algae of the Ostreopsis genus blooms in Algeria during summer 2009.] ovata en Méditerranée Nord Occidentale, Analyse non ciblée des biotoxines marines dans les produits de la pêche, Vertical distribution of the toxic epibenthic dinoflagellates Ostreopsis cf. Batch summary table for purification of PLTX-group toxin after elution through LH20. In order to ensure food safety and avoid exposure to these substances, national and international regulatory agencies have established tolerance levels for authorized veterinary drugs, including tetracycline antimicrobials. ovata strain, Chemical Ecology of the Benthic Dinoflagellate Genus Ostreopsis: Review of Progress and Future Directions, Harmful Algal Blooms: Effect on Coastal Marine Ecosystems, The dual impact of Ostreopsis cf. Ostreopsis ovata a fait quelques dégâts durant l'été 2008 sur les plages de la Côte d'Azur en provocant toux, fièvres et troubles respiratoires. The widely accepted recognition of the dependence of important cellular events on calcium ion concentration propelled us to investigate the occurrence of palytoxin-Ca2+ complex in aqueous solution by NMR- and Molecular Modeling-based approach. Le traitement est connu, et relativement simple : "Il faut des anti-inflammatoires, soigner la zone contaminée, traiter les gens qui ont des difficultés respiratoires comme une crise d'asthme". ovata, Prorocentrum lima and Coolia monotis) Developing in the Southern Mediterranean Basin, Ostreopsis cf. We developed a method to quantify VDBG with LC-MS/MS that could also identify the haplotypes/isoforms of VDBG present. Explications ↘ La Lettre d'information de l'Institut océanographique Paul Ricard - N°14 - 2015 . Factors considered likely to be responsible for the increasing trend in cases are the increased population size of the elderly and susceptible population except for the 25–44 female age group. Consultez le profil complet sur LinkedIn et découvrez les relations de Cécile, ainsi que des emplois dans des entreprises similaires. ovata isolated from Todos os Santos Bay, tropical southwestern Atlantic, On dinoflagellate phylogeny and classification, Behavioural trait of Ostreopsis ovata (Dinophyceae) in Mediterranean rock pools: The spider’s strategy, MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Toxic harmful algal bloom (HAB) occurrence is becoming more frequent and problematic in highly urbanized coastal zones. - Prélèvement des aérosols et traitement chimique. Par Sciences et Avenir avec AFP le 09.08.2021 à 17h57, mis à jour le 10.08.2021 à 15h12 Lecture 3 min. The polymers and their intermediates were characterized by means of 1H NMR spectroscopy and size exclusion chromatography. The efficacy of the method is exemplified by comparison of the results of the quantification obtained by LC–FLD and the hemolytic assay performed for palytoxins for which a highly significant linear correlation was achieved (r2 = 0.9118). Spearman’s analysis showed significant positive correlations between the β2-encoding ATP1B2 gene expression and Emax values (rho = 0.30; p = 0.025) and between Emax and the ATP1B2/ATP1B3 expression ratio (rho = 0.38; p = 0.004), as well as a significant negative correlation between Emax and the ATP1B1/ATP1B2 expression ratio (rho = −0.30; p = 0.026). Blooms of the toxic dinoflagellates Ostreopsis have become common along rocky shores of the Mediterranean Sea. Consultez le profil de Pauline Guibaud sur LinkedIn, le plus grand réseau professionnel mondial. Face à l’expansion géographique des biotoxines marines, à l’émergence de nouvelles toxines et compte tenu du risque avéré pour la santé humaine, il est essentiel de disposer d’outils suffisamment versatiles et performants pour détecter une gamme, la plus large possible, de toxines connues ou émergentes de manière à garantir la sécurité des consommateurs.
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